切换至 "中华医学电子期刊资源库"

中华医学超声杂志(电子版) ›› 2020, Vol. 17 ›› Issue (01) : 70 -75. doi: 10.3877/cma.j.issn.1672-6448.2020.01.013

所属专题: 文献

基础研究

经颅超声对电针治疗帕金森病模型大鼠的效果评价
王静1, 王安龙2, 范小明1, 王嶒1, 孙婷婷1, 李立红3,()   
  1. 1. 310014 杭州,浙江省人民医院超声科
    2. 310003 杭州市红十字会医院放射科
    3. 310014 杭州,浙江省人民医院针灸推拿科
  • 收稿日期:2018-11-06 出版日期:2020-01-01
  • 通信作者: 李立红
  • 基金资助:
    浙江中医药科技计划项目(2018ZQ005,2019ZQ035); 浙江省医药卫生科技项目(2019309786); 浙江省自然基金项目(S19H090006)

Transcranial ultrasonography for assessment of hyperechogenicity in the substantia nigra of rats with Parkinson's disease treated by electro-acupuncture

Jing Wang1, Anlong Wang2, Xiaoming Fan1, Ceng Wang1, Tingting Sun1, Lihong Li3,()   

  1. 1. Department of Ultrasound, Zhejiang Provincial People′s Hospital, Pelople′s Hospital of Hangzhou Medical College, Hangzhou 310014, China
    2. Department of Acupuncture, Hangzhou Red Cross Hospital, Hangzhou 310003, China
    3. Department of Radiology, Zhejiang Provincial People′s Hospital, Pelople′s Hospital of Hangzhou Medical College, Hangzhou 310014, China
  • Received:2018-11-06 Published:2020-01-01
  • Corresponding author: Lihong Li
  • About author:
    Corresponding author: Li Lihong, Email:
引用本文:

王静, 王安龙, 范小明, 王嶒, 孙婷婷, 李立红. 经颅超声对电针治疗帕金森病模型大鼠的效果评价[J/OL]. 中华医学超声杂志(电子版), 2020, 17(01): 70-75.

Jing Wang, Anlong Wang, Xiaoming Fan, Ceng Wang, Tingting Sun, Lihong Li. Transcranial ultrasonography for assessment of hyperechogenicity in the substantia nigra of rats with Parkinson's disease treated by electro-acupuncture[J/OL]. Chinese Journal of Medical Ultrasound (Electronic Edition), 2020, 17(01): 70-75.

目的

采用经颅超声(TCS)观察6-OHDA诱导的PD模型大鼠电针治疗前后中脑黑质区高回声(SNH)面积,结合黑质酪氨酸羟化酶(TH)免疫组化及血清、十二指肠和黑质炎症因子IL-1β和TNF-α等,探讨TCS在PD诊断和疗效评价中的应用价值。

方法

60只雄性SD大鼠,随机分为假手术组15只,6-OHDA造模45只,通过向黑质区单侧输注6-OHDA诱导PD模型,模型成功33只,随机分为模型组(16只)和电针组(17只),并于造模后第15 d和45 d进行行为学评估及超声观察,造模后第45 d,每组选取4只大鼠进行脑组织免疫组化染色,余下大鼠取心脏血后,根据解剖位置迅速分离出黑质及十二指肠,进行ELISA法检测,采用方差分析及t检验比较各组间的指标差异。

结果

造模15 d后的大鼠损伤侧黑质区均出现明显的大面积片状SNH;超声观察第45 d时电针组损伤侧SNH面积比模型组减少,差异有统计学意义(t=4.01,P<0.05);电针组TH阳性面积与模型组相比显著增加,差异有统计学意义(t=2.84,P<0.05);电针组血清和十二指肠TNF-α及十二指肠IL-1β较模型组有所下降,差异均有统计学意义(t=8.88,7.64、10.86,P均<0.05);模型组、电针组SNH面积与TH阳性面积之间存在显著相关性(r=-0.698、-0.723,P<0.05)。

结论

TCS可以成功观测PD模型大鼠电针治疗前后SNH的面积变化,可作为PD诊断和疗效评价的指标之一。

Objectives

To assess the substantia nigra (SN) hyperechogenicity (SNH) of 6-hydroxydopamine (6-OHDA)-induced Parkinson's disease (PD) rats before and after electro-acupuncture treatment by transcranial sonography (TCS), detect the expression of tyrosine hydroxylase (TH) in the SN by immunohistochemistry and the changes of inflammatory factors IL-1β and TNF-α in serum, duodenum, and SN, and explore the value of TCS in the diagnosis and treatment of PD.

Methods

Sixty male SD rats were randomly divided into either a sham-operation group (15 rats) or a 6-OHDA group (45 rats). PD was induced by injecting 6-OHDA unilaterally into the SN. A total of 33 models were successfully established. They were randomly divided into a model group (16 rats) and an electro-acupuncture group (17 rats). Behavioral and ultrasonographic assessments were carried out on the 15th and 45th days after modeling. On the 45th day, four rats in each group were selected for immunohistochemical staining for TH, and the rest of the rats were used to collect heart blood and SN, and duodenum tissues for detection of IL-1β and TNF-α by ELISA. ANOVA and t-test were used to compare the differences among and between groups.

Results

SNH was observed in the injured SN of the model rats. After treatment, the area of injured SNH in the electro-acupuncture group was significantly less than that in the model group (t=4.01, P<0.05). TH positive area in the electro-acupuncture group was significantly increased compared with the model group (t=2.84, P<0.05). The levels of TNF-α in the serum and duodenum, IL-1β in the duodenum in the electro-acupuncture group were significantly lower than those in the model group (t=8.88, 7.64, and 10.86, respectively, P<0.05).

Conclusion

TCS can be used to successfully observe the manifestations of SNH in PD rats before and after electro-acupuncture treatment, suggesting that TCS can be used as one of the evaluation indicators for the diagnosis and treatment of PD.

图1 超声图像与鼠脑图谱对照。红色标注区域即为黑质区域
表1 各组大鼠体质量比较(g,±s
表2 治疗前后各组大鼠旋转圈数比较(r/min,±s
表3 模型组与电针组大鼠治疗前后SNH面积比较(mm2±s
图2 经颅超声第45 d SNH情况图像。图示均为超声观察图像,图2a假手术组TCS未见明显SNH;图2b模型组显示较大面积的片状SNH(标记区域,面积约2.8 mm2);图2c电针组显示SNH(标记区域,面积约1.5 mm2
图3 酪氨酸羟化酶免疫组化染色图(×40)。图3a为假手术组;图3b为模型组;图3c为电针组
表4 模型组与电针组大鼠促炎因子比较(ng/L,±s
1
Martinez-Fernandez R, Schmitt E, Martinez-Martin P. The hidden sister of motor fluctuations in Parkinson′s disease: a review on nonmotor fluctuations [J]. MovDisord, 2016, 31(8): 1080-1094.
2
陈伶利,李杰,李新华, 等. 针刺筋会穴阳陵泉对帕金森模型小鼠黑质TH和GFAP表达的影响 [J]. 中西医结合心脑血管病杂志, 2011, 9(4): 464-466.
3
Belaidi AA, Bush AI. Iron neurochemistry in Alzheimer′s disease and Parkinson′s disease: targets for therapeutics [J]. J Neurochem, 2016, 139 Suppl 1: 179-197.
4
Guan X, Xuan M, Gu Q, et al. Regionally progressive accumulation of iron in Parkinson′s disease as measured by quantitative susceptibility mapping [J]. NMR Biomed, 2017, 30(4). Doi: 10.1002/nbm.3489.
5
马骏,王彦春,甘水咏. 电针风府穴,太冲穴对PD大鼠黑质神经元凋亡的影响 [J]. 湖北中医杂志, 2003, 25(11): 5-6.
6
Chen H, Zhao EJ, Zhang W, et al. Meta-analyses on prevalence of selected Parkinson′s non-motor symptoms before and after diagnosis [J]. Transl Neurodegener, 2015, 4(1): 1.
7
章建权,曾含漪. 帕金森病相关基因的研究进展 [J]. 实用临床医学, 2013, 14(5): 135-138.

URL    
8
胡景霞,白波. 脑-肠-微生物群轴与帕金森病 [J]. 济宁医学院学报, 2016, 4(39): 77-81.
9
Wirdefeldt K, Adami HO, Cole P, et al. Epidemiology and etiology of Parkinson′s disease: a review of the evidence [J]. Eur J Epidemiol, 2011, 26(Suppl 1): S1-S58.

URL    
10
Becker G, Seufert J, Bogdahn U, et al. Degeneration of substantia nigra in chronic Parkinson′s disease visualized by transcranial color-coded real-time sonography [J]. Neurology, 1995, 45(1): 182-184.
11
Walter U, Dressler D, Probst T, et al. Transcranial brain sonography findings in discriminating between parkinsonism and idiopathic Parkinson disease [J]. Arch Neurol, 2007, 64(11): 1635-1640.
12
Pilotto A, Yilmaz R, Berg D. Developments in the role of transcaranial sonography for the differential diagnosis of parkinsonism [J]. Curr Neurol Neurosci Rep, 2015, 15(7): 1-10.
13
肖芳,张迎春,盛余敬, 等. 帕金森病与肌张力障碍患者经颅超声特点分析 [J]. 中华医学杂志, 2015, 95(15): 1135-1137.
[1] 朱金伟, 陆件, 宁文娜, 陈亚欧. 基于肠道生物标志物探讨三种营养策略对重度胃肠损伤患者的价值[J/OL]. 中华危重症医学杂志(电子版), 2024, 17(02): 111-117.
[2] 刘清, 汪志凌. 肠道真菌与儿童炎症性肠病[J/OL]. 中华妇幼临床医学杂志(电子版), 2024, 20(02): 172-178.
[3] 王浩元, 王舒, 王娟, 杨建军. 基于类器官模型探索肠道与肠道菌群间相互关系的研究进展[J/OL]. 中华普通外科学文献(电子版), 2024, 18(03): 220-224.
[4] 杜彦斌, 黄涛, 寇天阔, 石英. 双镜联合根治术与腹腔镜根治术在早期结肠癌患者中的应用效果[J/OL]. 中华普外科手术学杂志(电子版), 2024, 18(03): 275-278.
[5] 邢益民, 张天飞, 戴慧勇. 胃肠充盈超声造影检查在反酸、嗳气患者临床诊断中的应用[J/OL]. 中华普外科手术学杂志(电子版), 2024, 18(03): 303-306.
[6] 李维坤, 邵欣欣, 胡海涛, 卢一鸣, 王鹏, 杜永星, 徐泉, 田艳涛. 腹腔镜胃间质瘤手术切除策略分析[J/OL]. 中华腔镜外科杂志(电子版), 2024, 17(03): 141-145.
[7] 张艺, 任秀君, 郭孟玮, 赵雅芳, 李一凡, 李佳阳, 任晓暄, 邬继红, 卢海洋. 电针预处理对脑缺血再灌注大鼠行为学及外周血内皮祖细胞的影响[J/OL]. 中华神经创伤外科电子杂志, 2024, 10(02): 71-77.
[8] 孙阳, 杨帅, 贾晋瑄, 杜震, 周凌峰, 魏贤振. 电针联合等速训练对THA术后髋关节生物力学的影响[J/OL]. 中华老年骨科与康复电子杂志, 2024, 10(02): 103-110.
[9] 李苒, 姜宇航, 陈泽浩, 何家恺, 闫珊珊, 鄢锦荣, 贾宝辉. 电针治疗阿尔茨海默病患者的先导性随机对照试验[J/OL]. 中华脑科疾病与康复杂志(电子版), 2024, 14(04): 218-224.
[10] 曾倩, 徐朝阳, 张丽芳. 帕金森病步态分析的研究进展[J/OL]. 中华脑科疾病与康复杂志(电子版), 2024, 14(04): 235-238.
[11] 邱岭, 朱旭丽, 浦坚, 邢苗苗, 吴佳玲. 糖尿病肾病患者肠道菌群生态特点与胃肠道功能障碍的关联性研究[J/OL]. 中华消化病与影像杂志(电子版), 2024, 14(05): 453-458.
[12] 马虹宇, 陈元武, 张明明, 岳向勇, 檀碧波. 平胃散加味联合毫火针对直肠癌患者化学治疗致胃肠道反应的疗效[J/OL]. 中华消化病与影像杂志(电子版), 2024, 14(04): 297-300.
[13] 赵倩, 刘文超, 李玺琳, 章邱东. 老年急性脑梗死诱发胃肠损伤的风险因素分析及模型构建[J/OL]. 中华消化病与影像杂志(电子版), 2024, 14(03): 213-217.
[14] 杨星, 刘梅, 刘思远, 张科. 胃肠癌患者术前焦虑情绪和术后苏醒期躁动情况及影响因素分析[J/OL]. 中华消化病与影像杂志(电子版), 2024, 14(02): 159-163.
[15] 蔡雨玲, 王刚, 江志伟. 针刺应用于术后肠麻痹的研究进展[J/OL]. 中华针灸电子杂志, 2024, 13(04): 164-168.
阅读次数
全文


摘要