To prepare a high molecular contrast agent carrying paclitaxel and anti human epidermal growth factor receptor-2 (HER-2) antibody, and to investigate the target ability in vitro and its effects on ultrasonography in vitro.

The paclitaxel-loaded and anti HER-2 antibodies polymer contrast agents were prepared by the double emulsion technique and carbodiimide technique, and its general properties were by tested. The combination of drug-loading targeted contrast agent and SKBR-3 breast cancer cells was observed with laser confocal microscope. The effect of imaging in vitro was observed by the high frequency ultrasonography.

The average diameter of drug-loaded targeting carboxyl terminal lactic acid (PLGA) nanoparticle was (318.07±12.51) nm, the surface potential was (-2.16±0.31) mV, the drug encapsulation efficiency was (60.00±3.40)%, the drug loading amounts was (6.00±0.34)%, and the contrast agent was spherical particles with uniform size and good dispersion. Laser confocal microscope found significant combination of anti HER-2 antibody and carboxyl terminal lactic acid/hydroxyacetic acid copolymer (PLGA-COOH) polymer contrast agent. Flow cytometry observed the combination rate was (99.59±0.42)%. Search target experiment in vitro observed drug targeting macromolecular contrast agent and SBKR-3 breast cancer cells had a high binding rate. In vitro imaging experiments showed that drug targeting macromclecular imgaging agent appeared uniform and fine dotted dense hyper-echogenicity.

A kind of her-2-targeted and paclitaxel loaded high molecular contrast agent was successfully prepared. It had good performance, high drug loading amounts and entrapment efficiency. It and SKBR-3 breast cancer cells had a high binding rate. The external imaging was better.