To assess the clinical application of real-time ultrasound-guided "cross positioning" renal biopsy in primary hospitals.

The success rate, the number of glomeruli, and the incidence of postoperative complications in 105 patients who underwent "cross positioning" renal biopsy under real-time ultrasound guidance at the Department of Ultrasound of Chongming Branch of Shanghai Xinhua Hospital from January 2018 to December 2019 were analyzed retrospectively.

In the 105 cases of renal biopsy included, more than ten glomeruli were obtained except one case in whom fat tissue was obtained. The success rate of renal biopsy was 99% (104/105). Twenty-five (23.8%, 25/105) cases of perirenal hematoma and subcapsular hematoma were detected by ultrasound, including five cases with hematoma larger than 25 mm and seven (6.7%, 7/105) with gross hematuria; the total complication rate was 30.5% (32/105).

It is safe and effective to carry out "cross positioning" renal biopsy under the guidance of real-time ultrasound in primary hospitals.