探讨Sonazoid^®超声造影（CEUS）在不同造影剂剂量浓度、机械指数（MI）和造影模式下的成像效果。

采用医用超声耦合剂和乳胶软管制备体外模型。Sonazoid^®超声造影剂稀释混匀备用。在不同造影模式[振幅调制成像（Gen）和脉冲相位反转成像（Hres1）]下，每一剂量浓度组（0.1、0.15、0.2 ml/L）分别在9个MI值（0.16、0.19、0.22、0.25、0.27、0.33、0.4、0.5及0.7）条件下向乳胶软管内缓慢匀速推注Sonazoid^® 24 ml。探头固定扫查切面为乳胶管最大长轴切面。应用时间-强度曲线定量分析不同剂量浓度、不同造影模式及不同MI条件下，管内造影剂的增强强度、衰减程度和微泡增强持续时间。

增强强度在0.15 ml/L组高于0.1 ml/L组[（31.0±5.0）dB vs（28.7±5.0）dB，P<0.001]，而衰减程度差异无统计学意义[1.0（0.3，1.8）dB vs 0.6（0.2，1.4）dB，P=0.064]。Hres1的增强强度及衰减程度大于Gen模式[（33.4±5.4）dB vs（28.9±4.5）dB，P<0.001；1.0（0.4，2.1）dB vs 0.8（0.3，1.7）dB，P=0.027]，与Gen模式相比，Hres1模式微泡增强持续时间更短[（6.9±1.8）s vs（7.5±2.5）s，P=0.003]。Hres1模式下MI=0.25及Gen模式下MI=0.22时可以较好平衡微泡增强持续时间和增强强度。

Sonazoid^®-CEUS的临床应用需考虑造影剂剂量浓度、MI、造影模式等因素的影响，以达到理想的成像效果。

To investigate the influence of dose, mechanical index (MI), and imaging techniques on contrast-enhanced ultrasound (CEUS) with Sonazoid^®.

In vitro models were prepared utilizing medical ultrasonic couplants and latex hoses. Sonazoid^® was diluted and prepared. In each dose group (0.1 ml/L, 0.15 ml/L, and 0.2 ml/L), Sonazoid^® (24 ml) was injected slowly and uniformly into the tube at 9 MI values (0.16, 0.19, 0.22, 0.25, 0.27, 0.33, 0.4, 0.5, and 0.7) using different imaging techniques [power modulation imaging (Gen) and pulse inversion imaging (Hres1)]. The probe was fixed to the maximum long-axis section of the latex hose for scanning. Quantitative analysis was performed using time-intensity curves to assess the enhancement, attenuation, and sustained enhancement time of microbubbles in the tube under different doses, MI values, and imaging techniques.

The enhancement in the 0.15 ml/Lgroup was higher than that of the 0.1 ml/L group [(31.0±5.0) dB vs (28.7±5.0) dB, P<0.001], but there was no significant difference in attenuation between them [1.0 (0.3, 1.8) dB vs 0.6 (0.2, 1.4) dB, P=0.064]. The enhancement and attenuation of Hres1 were higher than those of Gen [(33.4±5.4) dB vs (28.9±4.5) dB, P<0.001; 1.0 (0.4, 2.1) dB vs 0.8(0.3, 1.7) dB, P=0.027], and the sustained enhancement time of microbubbles was shorter [(6.9±1.8) s vs (7.5±2.5) s, P=0.003]. The balance between the enhancement and sustained enhancement time of microbubbles was obtained at MI=0.25 in Hres1 and MI=0.22 in Gen, respectively.

The clinical application of CEUS with Sonazoid^® should consider the influence of dose, MI, and imaging technique, in order to achieve ideal imaging effects.