Expression difference of factors CD36, NR4A1 and HES1 which were before and after the ethanol sclerotherapy for ovarian endometrial cyst detected by gene chips

doi:10.3877/cma.j.issn.1672-6448.2015.04.015

Abstract

Abstract:

Objective

To explore changes of immune factors in serum before and after ultrasound-guided (US-guided ) interventional treatment of ovarian endometrial cyst.

Methods

Nine patients with ovarian endometrial cyst were selected between December 2011 and December 2013 in the fourth affiliated hospital of Harbin Medical University. All patients performed US-guided interventional treatment, the serum specimens were collected before and after three months of treatment respectively. NimbleGen genome microarray of serum specimens were performed to analysis the gene function and gene pathways of related factors of ovarian endometrial cyst and to find out factors with significant expression differences before and after treatment. The relevance between factors with significant expression differences and the pathogenesis of ovarian endometrial cyst were analyzed.

Results

The expression of CD36, NR4A1and HES1 were found to be down-regulated after US-guided interventional treatment of ovarian endometrial cyst by NimbleGen genome microarray. The gene function of CD36, NR4A1 and HES1participating in angiogenesis, chemotaxis, cell adhesion was relevant to the pathogenesis of the ovarian endometrial cyst, and played roles through Adipocytokine signaling pathway, MAPK signalig pathway, Maturity onset diabetes of the young signaling pathways.

Conclusion

Serum CD36, NR4A1 and HES1of ovarian endometrial cyst were down-regulated after US-guided ethanol sclerotherapy , and involved in the pathogenesis of ovarian endometrial cyst .

Key words: Ovarian cysts, Uterus, Cysts, Ultrasonography, interventional, Immunologic factors

Ning Zhao, Xiaoqiu Dong, Lulu Wang, Siming Wang, Xiaohui Shao, Liwei Zhang. Expression difference of factors CD36, NR4A1 and HES1 which were before and after the ethanol sclerotherapy for ovarian endometrial cyst detected by gene chips[J]. Chinese Journal of Medical Ultrasound (Electronic Edition), 2015, 12(04): 330-334.

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URL: https://chaosheng.cma-cmc.com.cn/EN/10.3877/cma.j.issn.1672-6448.2015.04.015

https://chaosheng.cma-cmc.com.cn/EN/Y2015/V12/I04/330

Figures/Tables 5

References 12

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通路编号	通路名称	P值	ES	因子条数	因子名称
hsa04010	促分裂原活化蛋白激酶信号通路	0.00036629	3.43618	10	CACNB1、DDIT3、DUSP2、DUSP4、DUSP5、ELK1、FOS、GADD45A、HSPA1A、NR4A1*
hsa00601	鞘糖脂生物合成	0.00229731	2.63878	3	B4GALT1、FUT1、FUT3
hsa05166	人T细胞白血病病毒感染	0.00528154	2.27724	8	DVL1、ELK1、ETS2、FOS、FOSL1、HLA-C、IL6、MYBL1
hsa04920	脂肪细胞因子通路	0.00538956	2.26845	4	CD36*、PPARA、PRKAB1、SOCS3
hsa05020	朊病毒通路	0.00541396	2.26649	3	ELK1、HSPA1A、IL6
hsa05168	单纯疱疹感染	0.01073924	1.96903	6	FOS、GTF2IRD1、HLA-C、IL6、PER1、SOCS3
hsa05134	军团杆菌病	0.01875945	1.72678	3	CXCL2、HSPA1A、IL6
hsa04320	背-腹轴形成	0.02498201	1.60237	2	CPEB1、ETS2
hsa04950	青春晚期糖尿病通路	0.02697634	1.56902	2	FOXA2、HES1*
hsa04141	内质网内蛋白质加工过程	0.02766641	1.55805	5	DDIT3、DNAJB1、ERN1、HSPA1A、PPP1R15A
hsa04115	P53信号通路	0.03262001	1.48652	3	GADD45A、PMAIP1、TNFRSF10B
hsa05164	甲型流感病毒	0.03298676	1.48166	5	DNAJB1、HSPA1A、IL6、SOCS3、TNFRSF10B
hsa04380	破骨细胞分化	0.04246594	1.37196	4	FOS、FOSL1、JUNB、SOCS3

通路编号	通路名称	P值	ES	因子条数	因子名称
hsa04010	促分裂原活化蛋白激酶信号通路	0.00036629	3.43618	10	CACNB1、DDIT3、DUSP2、DUSP4、DUSP5、ELK1、FOS、GADD45A、HSPA1A、NR4A1*
hsa00601	鞘糖脂生物合成	0.00229731	2.63878	3	B4GALT1、FUT1、FUT3
hsa05166	人T细胞白血病病毒感染	0.00528154	2.27724	8	DVL1、ELK1、ETS2、FOS、FOSL1、HLA-C、IL6、MYBL1
hsa04920	脂肪细胞因子通路	0.00538956	2.26845	4	CD36*、PPARA、PRKAB1、SOCS3
hsa05020	朊病毒通路	0.00541396	2.26649	3	ELK1、HSPA1A、IL6
hsa05168	单纯疱疹感染	0.01073924	1.96903	6	FOS、GTF2IRD1、HLA-C、IL6、PER1、SOCS3
hsa05134	军团杆菌病	0.01875945	1.72678	3	CXCL2、HSPA1A、IL6
hsa04320	背-腹轴形成	0.02498201	1.60237	2	CPEB1、ETS2
hsa04950	青春晚期糖尿病通路	0.02697634	1.56902	2	FOXA2、HES1*
hsa04141	内质网内蛋白质加工过程	0.02766641	1.55805	5	DDIT3、DNAJB1、ERN1、HSPA1A、PPP1R15A
hsa04115	P53信号通路	0.03262001	1.48652	3	GADD45A、PMAIP1、TNFRSF10B
hsa05164	甲型流感病毒	0.03298676	1.48166	5	DNAJB1、HSPA1A、IL6、SOCS3、TNFRSF10B
hsa04380	破骨细胞分化	0.04246594	1.37196	4	FOS、FOSL1、JUNB、SOCS3

因子名称	因子编码	P值	FC倍数	基因功能
CD36	BC008406	0.035185	2.3897486	细胞粘连、防御反应的正性调控相关、止血调控、血小板激活
HES1	BC039152	0.021746	2.1665425	血管化作用、上皮细胞生长、细胞粘连
NR4A1	NM_002135	0.002354	3.1305428	血管化作用、表皮生长因子受体相关作用、趋化作用

因子名称	因子编码	P值	FC倍数	基因功能
CD36	BC008406	0.035185	2.3897486	细胞粘连、防御反应的正性调控相关、止血调控、血小板激活
HES1	BC039152	0.021746	2.1665425	血管化作用、上皮细胞生长、细胞粘连
NR4A1	NM_002135	0.002354	3.1305428	血管化作用、表皮生长因子受体相关作用、趋化作用

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