Establishment of hepatocellular carcinoma model in mouse by interventional ultrasound

doi:10.3877/cma.j.issn.1672-6448.2015.04.016

Abstract

Abstract:

Objective

To study the feasibility and stability of hepatocellular carcinoma model established in mouse with the guidance of ultrasound, and to explore the value of ultrasonography in hepatocellular carcinoma model.

Methods

The type of H22 cells, whose concentration was 2×107/ml, were injected into the left liver of 30 mice with the guidance of ultrasound. After injection, the mice were examined by ultrasound on the tenth and twentieth day, and implemented laparotomy on the twentieth day. The tumors were examined pathologically. The formation rate and growth of tumors were evaluated, and the ultrasonic features of tumors were observed.

Results

The formation rate of implanted tumors was 96.7% (29/30), and the peritumoral blood flow could be detected. The diameters measured by ultrasound and explorative laparotomy were (8.4±0.3) mm and (8.6±0.2) mm, respectively (P>0.05). The pathological examination showed that the tumors were hepatocellular carcinoma.

Conclusion

Establishing mouse model of hepatocellular carcinoma by ultrasound-guided is simple and minimally invasive, and it provides an ideal model of high formation rate for the study of hepatocellular carcinoma.

Key words: Models, animal, Ultrasonography, interventional, Carcinoma, hepatocellular, Mice

Haichao Yang, Tianxiu Dong, Cunli Guo, Wenjing Zhu, Tingting An, Xiuhua Yang. Establishment of hepatocellular carcinoma model in mouse by interventional ultrasound[J]. Chinese Journal of Medical Ultrasound (Electronic Edition), 2015, 12(04): 335-338.

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URL: https://chaosheng.cma-cmc.com.cn/EN/10.3877/cma.j.issn.1672-6448.2015.04.016

https://chaosheng.cma-cmc.com.cn/EN/Y2015/V12/I04/335

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References 13

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