Contrast-enhanced ultrasound characteristics of papillary thyroid carcinoma and its relationship with microvessel density and microvessel area

doi:10.3877/cma.j.issn.1672-6448.2017.04.008

Abstract

Abstract:

Objective

To explore the correlation between quantitative parameters of blood perfusion with contrast-enhanced ultrasound (CEUS) and microvessel density (MVD), microvessel area (MVA) in papillary thyroid carcinoma (PTC). And to investigate the value of CEUS in evaluating the angiogenesis in PTC before operation.

Methods

Totally 69 cases of patients with papillary thyroid carcinoma were selected from April 2014 to October 2016 in the Affiliated Hospital of Qingdao University. The CEUS characteristics of 69 patients with papillary thyroid carcinoma confirmed by pathology were retrospectively analyzed. The patients were divided into three groups according to maximum diameter of lesions (<1 cm group, 1-2 cm group and >2.0 cm group), and two groups according to pathologic reports (neck lymph node metastatic and nonmetastatic groups). The blood perfusion parameters between or among different groups were evaluated by ″t″test or one-way ANOVA. Immunohistochemical staining were performed to evaluate the MVD, MVA in the surgical specimens, and the correlation of quantitative parameters with MVD, MVA were assessed by Spearman.

Results

(1) Peak Intensity (Peak), area under the curve (AUC), MVD and MVA of thyroid carcinoma were lower than the surrounding normal thyroid tissue (14.95±4.96 vs 22.67±6.11, 970.01±263.20 vs 1798.35±563.67, 118.91±31.32 vs 206.27±39.58, 8.58±2.68 vs 18.47±3.13), and the differences were statistically significant (t=-8.700, -11.061, -14.377 and -20.532, all P<0.05). (2)With the increase of the lesion′s maximum diameter, Peak, AUC, MVD and MVA increased, and the differences were statistically significant (t=0.000, 0.000, 0.000, 0.000; t=0.027, 0.044, 0.033, 0.000; t=0.027, 0.044, 0.033, 0.000, all P<0.05). (3) Papillary thyroid carcinoma with lymphatic involvement had significantly higher values of Peak, AUC, MVD and MVA than those without lymphatic involvement (16.86±4.36 vs 13.80±3.55, 1128.16±290.85 vs 874.39±192.27, 114.12±30.69 vs 103.67±22.19, 10.30±2.44 vs 7.54±2.29), and the differences were statistically significant (t=3.177, 4.366, 6.336 and 4.742, all P<0.05). (4) A positive correlation existed between the Peak, AUC and MVD, and the differences were statistically significant (r=0.506, 0.478, all P<0.05). Peak, AUC and MVA showed positive correlation, and the differences were statistically significant (r=0.648, 0.653, all P<0.05). TP, MTT and MVD, MVA showed no correlations (all P>0.05).

Conclusions

The values of Peak and AUC calculated from CEUS were correlated to MVD and MVA. CEUS may be used to evaluated the angiogenesis of PTC before operation. And CEUS is helpful for prediction of prognosis of PTC.

Key words: Thyroid neoplasms, Ultrasonography, Microvessels

Jing Chong, Yongmei Sun, Chunping Ning, Hongqiao Wang, Zongli Yang, Hai Dong. Contrast-enhanced ultrasound characteristics of papillary thyroid carcinoma and its relationship with microvessel density and microvessel area[J]. Chinese Journal of Medical Ultrasound (Electronic Edition), 2017, 14(04): 274-279.

/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://chaosheng.cma-cmc.com.cn/EN/10.3877/cma.j.issn.1672-6448.2017.04.008

https://chaosheng.cma-cmc.com.cn/EN/Y2017/V14/I04/274

Figures/Tables 6

References 17

[1]	姜珏，张红丽，黄丽丽, 等. 甲状腺腺瘤超声造影特征与病理微血管密度相关性[J]. 中华实用诊断与治疗杂志, 2014, 28(10):983-985.
[2]	董海英，李萍，宁春平, 等. 超声造影定量分析在甲状腺良恶性结节鉴别诊断中的应用价值[J/CD]. 中华医学超声杂志(电子版), 2013, 10(2):110-114.
[3]	Wu Q, Wang Y, Li Y, et al. Diagnostic value of contrast-enhanced ultrasound in solid thyroid nodules with and without enhancement [J]. Endocrine, 2016, 53(2):480-488.
[4]	Xia Y, Jiang YX, Dai Q, et al. Contrast-enhanced ultrasound of hepatocellular carcinoma: Correlation of washout time and angiogenesis [J]. Clin Hemorheol Microcirc, 2011, 48(4):265-273.
[5]	Weidner N, Semple JP, Welch WR, et al. Tumor angiogenesis and metastasis:correlation invasive breast carcinoma [J]. N Engl J Med, 1991, 324(1):1-8.
[6]	周琦，张红丽，黄丽丽, 等. 结节性甲状腺肿超声造影特征与病理微血管密度的相关性[J]. 临床超声医学杂志, 2014, 16(10):661-663.
[7]	Nguyen TB, Cron GO, Bezzina K, et al. Correlation of Tumor Immunohistochemistry with Dynamic Contrast-Enhanced and DSC-MRI Parameters in Patients with Gliomas [J]. AJNR Am J Neuroradiol, 2016, 37(12):2217-2223.
[8]	Lo GM, Al Zahrani H, Jang HJ, et al. Detection of Early Tumor Response to Axitinib in Advanced Hepatocellular Carcinoma by Dynamic Contrast Enhanced Ultrasound [J]. Ultrasound Med Biol, 2016, 42(6):1303-1311.
[9]	梁霞，杨淼，程智, 等. 超声造影对甲状腺单发实性结节良恶性的诊断价值[J]. 临床超声医学杂志, 2014, 16(2):98-100.
[10]	Zhou Q, Jiang J, Shang X, et al. Correlation of Contrast-Enhanced Ultrasonographic Features with Microvessel Density in Papillary Thyroid Carcinomas [J]. Asian Pac J Cancer Prev, 2014, 15(17):7449-7452.
[11]	Tian H, Wang Q. Quantitative analysis of microcirculation blood perfusion in patients with hepatocellular carcinoma before and after transcatheter arterial chemoembolisation using contrast-enhanced ultrasound [J]. Eur J Cancer, 2016, 68:82-89.
[12]	Wendl CM, Janke M, Jung W, et al. Contrast-enhanced ultrasound with perfusion analysis for the identification of malignant and benign tumours of the thyroid gland [J]. Clin Hemorheol Microcirc, 2015, 63(2):113-121.
[13]	Bartolotta TV, Midiri M, Galia M, et al. Qualitative and quantitative evaluation of solitary thyroid nodules with contrast-enhanced ultrasound: initial results [J]. Eur Radiol, 2006, 16(10):2234-2241．
[14]	Zhuang PH, Xu L, Gao L, et al. Correlations of microvascular blood flow of contrast-enhanced ultrasound and HGF/c-Met signaling pathway with clinicopathological features and prognosis of patients with hepatocellular carcinoma [J]. Onco Targets Ther, 2017, 10:847-857.
[15]	Zhan WW, Zhou P, Zhou JQ, et al. Differences in sonographic features of papillary thyroid carcinoma between neck lymph node metastatic andnonmetastatic groups [J]. J Ultrasound Med, 2012, 31(6):915-920.
[16]	张波，姜玉新，戴晴, 等. 甲状腺实性结节超声造影与免疫组织化学分析[J]. 中国医学影像技术, 2011, 27(9):1783-1787.
[17]	Yildiz E, Ayan S, Goze F, et al. Relation of microvessel density with microvascular invasion, metastasis and prognosis in renal cell carcinoma [J]. BJU Int, 2008, 101(6):758-764.

取样位置	个数	TP（s）	MTT（s）	Peak（%）	AUC（%?s）	MVD（条/HP）	MVA（%）
PTC结节	69	43.69±17.92	62.14±18.10	14.95±4.96	970.01±263.20	118.91±31.32	8.58±2.68
正常组织	69	39.04±16.81	56.78±18.15	22.67±6.11	1798.35±563.67	206.27±39.58	18.47±3.13
t值	?	1.571	1.735	-8.700	-11.061	-14.377	-20.532
P值	?	0.118	0.085	<0.001	<0.001	<0.001	<0.001

取样位置	个数	TP（s）	MTT（s）	Peak（%）	AUC（%?s）	MVD（条/HP）	MVA（%）
PTC结节	69	43.69±17.92	62.14±18.10	14.95±4.96	970.01±263.20	118.91±31.32	8.58±2.68
正常组织	69	39.04±16.81	56.78±18.15	22.67±6.11	1798.35±563.67	206.27±39.58	18.47±3.13
t值	?	1.571	1.735	-8.700	-11.061	-14.377	-20.532
P值	?	0.118	0.085	<0.001	<0.001	<0.001	<0.001

肿瘤大小	个数	TP（s）	MTT（s）	Peak（%）	AUC（%?s）	MVD（条/HP）	MVA（%）
<1 cm组	26	44.59±14.72	61.66±14.82	12.77±3.14^ab	837.40±194.95^ab	102.15±24.69^ab	6.69±2.14^ab
1~2 cm组	29	45.48±20.09	62.65±22.43	14.88±3.58^c	960.46±206.13^c	118.00±25.12^c	9.03±2.14^c
>2 cm组	14	37.24±19.25	61.99±14.32	19.16±3.71	1236.08±293.53	151.93±34.22	11.16±2.11
F值	?	0.956	0.021	15.643	14.688	15.485	21.140
P值	?	0.390	0.979	<0.001	<0.001	<0.001	<0.001

肿瘤大小	个数	TP（s）	MTT（s）	Peak（%）	AUC（%?s）	MVD（条/HP）	MVA（%）
<1 cm组	26	44.59±14.72	61.66±14.82	12.77±3.14^ab	837.40±194.95^ab	102.15±24.69^ab	6.69±2.14^ab
1~2 cm组	29	45.48±20.09	62.65±22.43	14.88±3.58^c	960.46±206.13^c	118.00±25.12^c	9.03±2.14^c
>2 cm组	14	37.24±19.25	61.99±14.32	19.16±3.71	1236.08±293.53	151.93±34.22	11.16±2.11
F值	?	0.956	0.021	15.643	14.688	15.485	21.140
P值	?	0.390	0.979	<0.001	<0.001	<0.001	<0.001

组别	例数	TP（s）	MTT（s）	Peak（%）	AUC（%?s）	MVD（条/HP）	MVA（%）
淋巴结转移组	26	43.74±19.38	64.99±17.31	16.86±4.36	1128.16±290.85	144.12±30.69	10.30±2.44
淋巴结未转移组	43	43.66±17.21	60.41±18.55	13.80±3.55	874.39±192.27	103.67±22.19	7.54±2.29
t值	?	0.016	1.020	3.177	4.366	6.336	4.742
P值	?	0.987	0.312	0.002	<0.001	<0.001	<0.001

Please choose a citation manager

Content to export