Apoptosis induced by low-intensity ultrasound combined with microbubbles in human gallbladder cancer GBC-SD cells

doi:10.3877/cma.j.issn.1672-6448.2018.11.016

Abstract

Abstract:

Objective

To investigate the effect of low-intensity ultrasound combined with microbubbles on apoptosis of human gallbladder cancer GBC-SD cells.

Methods

Ultrasound with a frequency of 1 MHz and intensity of 0.45 W/cm² in continuous wave mode was used. Human gallbladder cancer GBC-SD cells were divided into four groups, including control group (A, without any treatment), microbubbles group (B, mixed with 20% microbubbles), ultrasound group (C, low frequency ultrasonic continuous wave irradiation for 30 s) and combined group (D, microbubbles combined with ultrasonic waves). Cell proliferation activity of each group was detected by CCK-8 assay, and apoptosis was detected by flow cytometry.

Results

Proliferative activity of group A, B, C and D was 0.9272±0.1173, 1.0088±0.0628, 0.2116±0.0101 and 0.1470±0.0029, respectively. The proliferative activity of group D was lower than that of the other groups (t=14.846, 30.637 and 13.661, all P<0.05). Apoptosis was observed in all treated cells. Cell apoptosis rates of group D, A, B and C was 0.682±0.022, 0.073±0.005, 0.031±0.003 and 0.259±0.012, respectively. The apoptosis rate of group D was obviously higher than those of the other groups (F=2390.900, P<0.05).

Conclusion

Low-frequency ultrasound combined with microbubbles can reduce proliferative activity of human gallbladder cancer GBC-SD cells in vitro, and obviously induce the apoptosis of human gallbladder cancer GBC-SD cells.

Key words: Ultrasonography, Contrast media, Gallbladder neoplasms, Apoptosis, Cell proliferation

Liying Wang, Shusen Zheng. Apoptosis induced by low-intensity ultrasound combined with microbubbles in human gallbladder cancer GBC-SD cells[J]. Chinese Journal of Medical Ultrasound (Electronic Edition), 2018, 15(11): 874-878.

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URL: https://chaosheng.cma-cmc.com.cn/EN/10.3877/cma.j.issn.1672-6448.2018.11.016

https://chaosheng.cma-cmc.com.cn/EN/Y2018/V15/I11/874

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