Establishment of an animal model of iliac artery hemorrhage under ultrasound guidance

doi:10.3877/cma.j.issn.1672-6448.2021.04.012

Abstract

Abstract:

Objective

To explore the feasibility of establishing an animal model of iliac artery hemorrhage under ultrasound guidance.

Methods

The experimental animals used in this study were nine male Wuzhishan pigs with a mean weight of (14.06±0.31) kg. Limited fluid resuscitation and application of anesthetic through an internal jugular vein indwelling catheter were given. Invasive arterial blood pressure (ABP) and heart rate (HR) were recorded using a biological signal collection and processing system connected with a pressure transducer via the arteria carotis connect. Under the guidance of ultrasound, a disposable puncture biopsy device was used to puncture one side of the iliac artery. The changes in the luminal shape and hemodynamics of the iliac artery and the volume of retroperitoneal blood accumulation were observed by ultrasound and color Doppler ultrasound. Thirty minutes after hemorrhage, the animals were sacrificed for anatomy, and the specimens of both sides of the iliac artery were collected.

Results

The rupture of the iliac artery occurred in all nine experimental animals. After puncturing the iliac artery, blood pressure fell [systolic pressure: (120.33±16.69) mmHg vs (84.56±13.43) mmHg, P＜0.05; diastolic pressure: (75.11±16.57) mmHg vs (59.44±19.01) mmHg, P＜0.05] and heart rate increased [(77.89±22.98) bpm vs (111.33±26.182) bpm, P＜0.05), which was consistent with hemorrhagic shock. The artery rapidly collapsed and the inner diameter of the artery narrowed [(0.39±0.63) cm vs (0.22±0.54) cm, P＜0.05]. The maximum depth of ascites increased gradually [(0.13±0.12) cm vs (3.03±0.99) cm, P＜0.05]. After the animals were sacrificed and the abdomen was opened, huge blood clots in the abdominal and retroperitoneum were visible. The gross pathological findings confirmed the rupture of the right iliac artery.

Conclusion

It is feasible to establish an animal model of iliac artery hemorrhage under the guidance of ultrasound. This method is simple and has a high success rate.

Key words: Ultrasonography, Iliac artery, Hemorrhage, Swine, Animal model, Punctures

Yuqing Huang, Shengzheng Wu, Xianghui Chen, Xuexia Shan, Xingxi Lin, Liye Zhang, Xuan Zhou, Faqin Lyu. Establishment of an animal model of iliac artery hemorrhage under ultrasound guidance[J]. Chinese Journal of Medical Ultrasound (Electronic Edition), 2021, 18(04): 407-411.

/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://chaosheng.cma-cmc.com.cn/EN/10.3877/cma.j.issn.1672-6448.2021.04.012

https://chaosheng.cma-cmc.com.cn/EN/Y2021/V18/I04/407

Figures/Tables 5

References 13

1	2017 DALYs GBD and HALE Collaborators. Global, regional, and national disability-adjusted life-years (DALYs) for 359 diseases and injuries and healthy life expectancy (HALE) for 195 countries and territories, 1990-2017: a systematic analysis for the Global Burden of Disease Study 2017 [J]. Lancet, 2018, 392(10159): 1859-1922.
2	Tremoleda JL, Watts SA, Reynolds PS, et al. Modeling acute traumatic hemorrhagic shock injury: challenges and guidelines for preclinical studies [J]. Shock, 2017, 48(6): 610-623.
3	Chang R, Fox EE, Greene TJ, et al. Multicenter retrospective study of non-compressible torso hemorrhage: anatomic locations of bleeding and comparison of endovascular versus open approach [J]. Trauma Acute Care Surg, 2017, 83(1): 11-18.
4	Kotwal RS, Butler FK, Gross KR, et al. Management of junctional hemorrhage in tactical combat casualty care: TCCC Guidelines?Proposed Change 13-03 [J]. J Spec Oper Med, 2013, 13(4): 85-93.
5	Bennett BL, Butler FK, Wedmore IS. Tactical combat casualty care: transitioning battlefield lessons learned to other austere environments [J]. Wilderness Environ Med, 2017, 28(2S): S3-S4.
6	van Oostendorp SE, Tan ECTH, Geeraedts Jr LMG. Prehospital control of life-threatening truncal and junctional haemorrhage is the ultimate challenge in optimizing trauma care: a review of treatment options and their applicability in the civilian trauma setting [J]. Scand J Trauma Resusc Emerg Med, 2016, 24(1): 110.
7	Eastridge BJ, Mabry RL, Seguin P, et al. Death on the battlefield (2001-2011): implications for the future of combat casualty care [J]. J Trauma Acute Care Surg, 2012, 73(6 Suppl 5): S431-S437.
8	Arnaud F, Parreño-Sadalan D, Tomori T, et al. Comparison of 10 hemostatic dressings in a groin transection model in swine [J]. Trauma, 2009, 67(4): 848-855.
9	Kheirabadi BS, Terrazas IB, Miranda N, et al. Physiological consequences of abdominal aortic and junctional tourniquet (AAJT) application to control hemorrhage in a swine model [J]. Shock, 2016, 46(3 Suppl 1): 160-166.
10	Lee C, Balk D, Schafer J, et al. Accuracy of focused assessment with sonography for trauma (FAST) in disaster settings: a meta-analysis and systematic review [J]. Disaster Med Public Health Prep, 2019, 13(5-6): 1059-1064.
11	Todsen T, Tolsgaard MG. Assessment of competence in focused assessment with sonography for trauma (FAST) examination [J]. Trauma Acute Care Surg, 2016, 80(2): 353.
12	Lv F, Ning Y, Zhou X, et al. Effectiveness of contrast-enhanced ultrasound in the classification and emergency management of abdominal trauma [J]. Eur Radiol, 2014, 24(10): 2640-2648.
13	吕发勤, 黄钰清, 刘义灏, 等. 超声技术在特殊环境的应用及其前景 [J/CD]. 中华医学超声杂志(电子版), 2019, 16(12): 885-888.

时间	数量（只）	收缩压（mmHg）	舒张压（mmHg）	心率（次/分）	腹腔积液量（cm）	髂动脉内径（cm）
建模前	9	120.33±16.69	75.11±16.57	77.89±22.98	0.13±0.12	0.39±0.63
建模后	9	84.56±13.43	59.44±19.01	111.33±26.18	3.03±0.99	0.22±0.54
t值		14.89	3.21	-5.30	-9.16	6.10
P值		0.00	0.01	0.01	0.00	0.02

时间	数量（只）	收缩压（mmHg）	舒张压（mmHg）	心率（次/分）	腹腔积液量（cm）	髂动脉内径（cm）
建模前	9	120.33±16.69	75.11±16.57	77.89±22.98	0.13±0.12	0.39±0.63
建模后	9	84.56±13.43	59.44±19.01	111.33±26.18	3.03±0.99	0.22±0.54
t值		14.89	3.21	-5.30	-9.16	6.10
P值		0.00	0.01	0.01	0.00	0.02

[1]	Shujie Wei, Pinjing Hui, Yafang Ding, Bai Zhang, Yanhong Yan, Peng Zhou, Yabo Huang. Evaluation of cerebral hemodynamics in patients with unilateral internal carotid artery occlusion undergoing superficial temporal artery-middle cerebral artery bypass [J]. Chinese Journal of Medical Ultrasound (Electronic Edition), 2023, 20(10): 1046-1055.
[2]	Xuan Zhang, Yutong Ma, Yuqian Miao, Yun Zhang, Shiwen Wu, Xiaochu Dang, Yingying Chen, Zhaoming Zhong, Xuejuan Wang, Miao Hu, Yanfeng Sun, Xiuzhu Ma, Faqin Lyu, Haiyan Kou. Ultrasound assessment of diaphragm function in pediatric patients with Duchenne muscular dystrophy [J]. Chinese Journal of Medical Ultrasound (Electronic Edition), 2023, 20(10): 1068-1073.
[3]	Lianhua Zhu, Xiang Fei, Peng Han, Bo Jiang, Nan Li, Yukun Luo. Value of high frame rate contrast-enhanced ultrasound in differential diagnosis of gallbladder polyp lesions [J]. Chinese Journal of Medical Ultrasound (Electronic Edition), 2023, 20(09): 904-910.
[4]	Meifang Zhang, Ying Tan, Qiaozhen Zhu, Xin Wen, Ying Yuan, Yue Qin, Hongbo Guo, Lingxiu Hou, Wenlan Huang, Guiyan Peng, Shengli Li. Artificial intelligence-based quality control of mid-sagittal plane ultrasound images for first trimester fetal crown-rump length [J]. Chinese Journal of Medical Ultrasound (Electronic Edition), 2023, 20(09): 945-950.
[5]	Ruina Zhao, Yuxin Jiang, Tao Chen, Li Zhang, Cheng Chen, Siqi Jin, Xueqi Chen, Xiao Yang, Jianchu Li. Present status and reform demands of continuing medical education for ultrasonic physicians in Beijing [J]. Chinese Journal of Medical Ultrasound (Electronic Edition), 2023, 20(09): 966-973.
[6]	Yuhan Zhou, Huan Xiao, Chunjiang Yang, Juan Zhou, Lirong Zhu, Juan Xu, Fangting Mou. Diagnostic value of ultrasound in children with temporary hip synovitis [J]. Chinese Journal of Medical Ultrasound (Electronic Edition), 2023, 20(08): 795-800.
[7]	Huanyan Liu, Yang Hua, Lingyun Jia, Xinyu Zhao, Beibei Liu. Lumen structure and hemodynamic characteristics of occluded internal carotid artery [J]. Chinese Journal of Medical Ultrasound (Electronic Edition), 2023, 20(08): 809-815.
[8]	Lianlian Zhang, Pinjing Hui, Yafang Ding. Clinical value of carotid Doppler ultrasonography in assessing vulnerability of atherosclerotic plaques [J]. Chinese Journal of Medical Ultrasound (Electronic Edition), 2023, 20(08): 816-821.
[9]	Yaping Jia, Shu'e Zeng. Ultrasound and pathological characteristics of breast metaplastic carcinoma containing squamous cell carcinoma components [J]. Chinese Journal of Medical Ultrasound (Electronic Edition), 2023, 20(08): 844-848.
[10]	Gang Luo, Silin Pan, Taotao Chen, Qian Xu, Zhixian Ji, Sibao Wang, Lingyu Sun. Application of echocardiography in fetal cardiac intervention of pulmonary atresia with intact ventricular septum [J]. Chinese Journal of Medical Ultrasound (Electronic Edition), 2023, 20(06): 605-609.
[11]	Qian Yang, Cuifang Li, Wanqiu Zhang. Analysis of short-term and long-term prognosis and influencing factors of primary liver cancer with spontaneous ruptured hemorrhage after emergency TACE surgery [J]. Chinese Journal of Operative Procedures of General Surgery(Electronic Edition), 2024, 18(01): 33-36.
[12]	Mei Liao, Hongjun Zhang, Jieyang Jin, Yan Lyu, Jie Ren. Diagnostic value of bedside contrast-enhanced ultrasound for early hepatic artery thrombosis after liver transplantation [J]. Chinese Journal of Hepatic Surgery(Electronic Edition), 2023, 12(06): 630-634.
[13]	Tianchi Yang, Wei Han, Feng Qiu, Jiahui Qi. Evaluation of pancreatic texture by intraoperative ultrasonic elastography [J]. Chinese Journal of Hepatic Surgery(Electronic Edition), 2023, 12(06): 646-650.
[14]	Fengyun Wu, Xin Teng, Lianjuan Liu. Diagnostic value of high-frequency ultrasonography and enhanced magnetic resonance for primary hyperdifferentiated hepatocellular carcinoma of different diameters [J]. Chinese Journal of Digestion and Medical Imageology(Electronic Edition), 2023, 13(06): 404-408.
[15]	Xinxin Sun, Jun Liu, Chaowu Chen, Chao Sun. Application of endoscopic ultrasonography-guided fine needle aspiration in pancreatic space-occupying lesions [J]. Chinese Journal of Digestion and Medical Imageology(Electronic Edition), 2023, 13(06): 418-421.

Please choose a citation manager

Content to export